Microglia and putative macrophages of the subfornical organ: structural and functional features

The subfornical organ is an important regulator of water-salt metabolism and energy balance the body, involved in control cardiovascular system immune regulation. comprises several cell populations, among which microglia macrophages remain uncharacterized. This study aimed at structural, cytochemical, functional characterization rats. Brain specimens were collected from mature male Wistar rats (n = 8). Microglia revealed by immunostaining with poly- monoclonal antibodies against calcium-binding protein Iba1 lysosomal CD68; slides examined light confocal laser microscopy. provides a comprehensive morphological microglial cells organ. We demonstrate that majority Iba1-expressing this area brain are cells, not macrophages. Pre-activated state may reflect structural features specific functions local microglia. Subependymal processes penetrate into third ventricle brain, constitute distinct subpopulation Apart elements, contains few tissue characteristic strong expression CD68 accompanied undetectable or weak Iba1.